Journal: Microorganisms

Article Title: Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

doi: 10.3390/microorganisms9071537

Figure Lengend Snippet: Primers sequences for qPCR.

Article Snippet: IGF2 , Rabbit , 1/500 , OAAB07463 , OAAB07463 , BosterBio , 20.

Techniques: Amplification

Journal: Microorganisms

Article Title: Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

doi: 10.3390/microorganisms9071537

Figure Lengend Snippet: Antibodies used for Western blot.

Article Snippet: IGF2 , Rabbit , 1/500 , OAAB07463 , OAAB07463 , BosterBio , 20.

Techniques: Western Blot

Journal: Microorganisms

Article Title: Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

doi: 10.3390/microorganisms9071537

Figure Lengend Snippet: Transcription factors and autoantigens transcripts in the whole thymus. Complementary DNA obtained from mock- and CV-B4-infected thymuses ( n = 5–7), harvested at different time-points, was submitted to quantification of Aire , Fezf2 , Igf2, and Myo7 transcripts by qPCR. Transcripts levels were normalized to those of Oaz1. Relative gene expression in CV-B4 E2- vs. mock-infected thymuses was calculated, as described in , and expressed as 100 × mean 2 −ΔΔCt values ± SD. The unpaired t -test was used for statistical analysis. ** p < 0.01. Mock (●) mock-infected thymuses harvested at different time points, from fetuses at day 17 of gestation (day 17G), and newborns at day 1 and day 5 from birth. CV-B4 10G (▼): thymus harvested from mice born to dams inoculated with CV-B4 at day 10 of gestation; CV-B4 17G (♦): thymus harvested from mice born to dams inoculated with CV-B4 at day 17 of gestation.

Article Snippet: IGF2 , Rabbit , 1/500 , OAAB07463 , OAAB07463 , BosterBio , 20.

Techniques: Immunopeptidomics, Infection, Gene Expression

Journal: Microorganisms

Article Title: Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

doi: 10.3390/microorganisms9071537

Figure Lengend Snippet: Transcription factors and autoantigens transcripts in enriched thymic epithelial cells. Whole thymuses were harvested, at different time-points, from offspring born to mock- and CV-B4-infected dams. Thymuses from fetuses or neonates of the same litter ( n = 5–12) were pooled and subjected to TECs isolation. Transcripts levels for Aire , Fezf2 , Igf2, and Myo7 , in enriched TECs, were determined by RT-qPCR and normalized to those of Oaz1. Relative gene expression in TECs from CV-B4 E2- vs. mock-infected thymuses was calculated, as described in , and expressed as 100 × mean 2 −ΔΔCt values ± SD. The unpaired t -test was used for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001. Mock (●) TECs from mock-infected thymuses harvested at different time points, from fetuses at day 17 of gestation (day 17G), and newborns at day 1 and day 5 from birth. CV-B4 10G (▼): TECs from thymus harvested from mice born to dams inoculated with CV-B4 at day 10 of gestation; CV-B4 17G (♦): thymus harvested from mice born to dams inoculated with CV-B4 at day 17 of gestation.

Article Snippet: IGF2 , Rabbit , 1/500 , OAAB07463 , OAAB07463 , BosterBio , 20.

Techniques: Immunopeptidomics, Infection, Isolation, Quantitative RT-PCR, Gene Expression

Journal: Microorganisms

Article Title: Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

doi: 10.3390/microorganisms9071537

Figure Lengend Snippet: Transcription factors and autoantigens protein expression in the whole thymus. ( a ) Representative blots depicting the expression levels of AIRE, FEZF2, IGF2, and MYO7 proteins in CV-B4- and mock-infected thymus sampled at day 1. ( b ) Relative expression of AIRE, FEZF2, IGF2, and MYO7 determined by Western blot analysis. Proteins obtained from mock- and CV-B4-infected thymuses ( n = 3–5), harvested at different time-points, were submitted to AIRE, FEZF2, IGF2, and MYO7 quantification by Western-blot analysis. Proteins levels were normalized to those of VINCULIN used as a loading control. Relative protein levels in CV-B4 E2- vs. mock-infected thymuses are represented as mean ± SD. The unpaired t -test was used for statistical analysis. * p < 0.05. Mock (●): mock-infected thymuses harvested at different time points, from fetuses at day 17 of gestation (day 17G), and newborns at day 1 and day 5 from birth. CV-B4 10G (▼): thymus harvested from mice born to dams inoculated with CV-B4 at day 10 of gestation; CV-B4 17G (♦): thymus harvested from mice born to dams inoculated with CV-B4 at day 17 of gestation.

Article Snippet: IGF2 , Rabbit , 1/500 , OAAB07463 , OAAB07463 , BosterBio , 20.

Techniques: Immunopeptidomics, Expressing, Infection, Western Blot, Control

Journal: Microorganisms

Article Title: Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

doi: 10.3390/microorganisms9071537

Figure Lengend Snippet: Relationship between viral load and transcription factors, Aire ( a ) and Fezf2 ( b ), and autoantigens transcripts, Igf2 ( c ) and Myo7 ( d ), in the thymus. For each harvested thymus (CV-B4-infected or age-matched control), transcription factors and autoantigens transcripts together with the corresponding viral load were measured and plotted in the same graph. To better assess the effect of infection on transcription factors and autoantigens expression, an eventual correlation between both parameters was evaluated using the Spearmen’s correlation test. r is the Spearman’s correlation coefficient. p -values were corrected according to the Benjamini–Hochberg procedure ( P BH ). Thymuses harvested at day 17G (Day 17G (10G) (●)), day 1 (Day 1 (10G) (▲)) or day 5 (Day 5 (10G) (♦)) from mice born to dams inoculated (or mock) with CV-B4 at day 10 of gestation; thymuses harvested at day 1 (Day 1 (17G) (▼)) or day 5 (Day 5 (17G) (■)) from mice born to dams inoculated (or mock) with CV-B4 at day 17 of gestation. x and y axis were log-scaled.

Article Snippet: IGF2 , Rabbit , 1/500 , OAAB07463 , OAAB07463 , BosterBio , 20.

Techniques: Immunopeptidomics, Infection, Control, Expressing

Journal: International journal of molecular sciences

Article Title: SNHG15-Mediated Localization of Nucleolin at the Cell Protrusions Regulates CDH2 mRNA Expression and Cell Invasion.

doi: 10.3390/ijms242115600

Figure Lengend Snippet: Figure 2. IMP1 regulates the localization of SNHG15 at the cell protrusions. (A) FISH was per- formed to detect the subcellular localization of SNHG15 in WT BT-549 cells (upper panel) and SNHG15-expressing BT-549 cells (lower panel). The arrowheads indicate localized SNHG15 at the cell protrusions. Scale bar: 10 µm. (B) FISH indicated that, in comparison with WT MDA-MB-231 cells (upper panel), IMP1 expression greatly increased the localization of SHNG15 at cell protrusions (lower panel). Scale bar: 10 µm. (C) A bar graph indicates the percentage of protrusion-localized SNHG15 in tested cells. Localization was increased to 70% from 40% when ectopic IMP1 was ex- pressed. About 80–100 cells were counted in each group. ** p < 0.01. WT: cell origin. IMP1: cells expressing ectopic IMP1. Control: cells transfected with an empty plasmid. (D) RT-qPCR showed that IMP1 expression does not affect cellular levels of SNHG15.

Article Snippet: Primary antibodies against IMP1, GAPDH and nucleolin (NCL) were purchased from Cell Signaling Co. (Danvers, MA, USA); CDH2 and CDH1 antibodies were purchased from Boster Biological Technology Co. (Wuhan, China).

Techniques: Expressing, Comparison, Control, Transfection, Plasmid Preparation, Quantitative RT-PCR

Journal: International journal of molecular sciences

Article Title: SNHG15-Mediated Localization of Nucleolin at the Cell Protrusions Regulates CDH2 mRNA Expression and Cell Invasion.

doi: 10.3390/ijms242115600

Figure Lengend Snippet: Figure 3. Localization of SNHG15 at the cell protrusions increases cell invasive potential. (A) The full-length SNHG15 and three dissected fragments of SNHG15 are separately tagged with six MS2 repeats. The relative position of the truncated SNHG15 is shown. The black box indicates the “ACACCC” motif for IMP1 binding. (B) FISH experiments showed that deletion of the “ACACCC” motif (Mut SNHG15) prevented SNHG15 from localizing at the cell protrusions. The arrowhead indicates cell-protrusion-localized SNHG15. Scale bar: 10 µm. (C) A bar graph shows that the cell population with protrusion-localized SNHG15 was largely decreased when the “ACACCC” motif was absent. ** p < 0.01. (D) Localization of the full-length and three truncated fragments of SNHG15 was determined by FISH. Results indicated that T1 and T3, but not T2, were still able to localize at the cell protrusions. About 80–100 cells were counted in each group. ** p < 0.01. (E) Transwell assays showed that, compared to the cells expressing full-length SNHG15, all three stable cell lines expressing truncated SNHG15 displayed lower invasive potentials. ** p < 0.01.

Article Snippet: Primary antibodies against IMP1, GAPDH and nucleolin (NCL) were purchased from Cell Signaling Co. (Danvers, MA, USA); CDH2 and CDH1 antibodies were purchased from Boster Biological Technology Co. (Wuhan, China).

Techniques: Binding Assay, Expressing, Stable Transfection

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The primers and TaqMan probes of IGF, ER genes used for RT-PCR

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques:

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The mRNA levels of IGF-1, IGF-1R, IGF-2, IGF-2R, IGFBP-3, ERα and ERβ in EAC, tumor-adjacent, and control groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Control

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The immunostaining of IGF-1R, IGF-2R, ERα and ERβ in EAC, atypical hyperplasia, and control groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Immunostaining, Control, Positive Control

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The relationship between the IGF and ER mRNA expression and clinico-pathological features of endometrioid adenocarcinoma

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The relationship between the IGF-1R, IGF2R, ERα and ERβ protein expression and clinico-pathological features of endometrioid adenocarcinoma

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: Correlation analysis of IGF and ER mRNA expression with clinico-pathological features of EAC

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: Correlation analysis of IGF-1R, IGF-2R, and ERα and ERβ protein expression with clinico-pathological features of EAC

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The analysis of the correlation between the mRNA expression of IGFs with ER subtypes in three groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing, Control

Journal: Bioactive Materials

Article Title: Translational selenium nanoparticles trigger apoptosis in triple-negative breast cancer cells through the MAPKs/Bcl2 pathway

doi: 10.1016/j.bioactmat.2026.02.027

Figure Lengend Snippet: Schematic illustration of PTR-SeNPs and MUC1@PTR-SeNPs synthesis and their anti-tumor efficacy against human triple-negative breast cancer.

Article Snippet: Anti-human MUC1 therapeutic antibody Fab fragment (7B8) were purchased From Creative Biolabs (TAB-423MZ-F, USA).

Techniques:

Journal: Bioactive Materials

Article Title: Translational selenium nanoparticles trigger apoptosis in triple-negative breast cancer cells through the MAPKs/Bcl2 pathway

doi: 10.1016/j.bioactmat.2026.02.027

Figure Lengend Snippet: Structure characterization of PTR-SeNPs and MUC1@ PTR-SeNPs. Structure characterization of PTR-SeNPs by (A) TEM, (B) Zetasizer Nano ZS, (C, D) Nanosight NS300, (E1-4) HRTEM-EDS and (F, G) FT-IR. (H) Confirmation of MUC1-C + PTR-SeNPs conjugation by confocal microscopy after fluorescent labeling with anti-mouse IgG (H + L). (I, J) Characterization results of the particle size and potential of MUC1@PTR-SeNPs

Article Snippet: Anti-human MUC1 therapeutic antibody Fab fragment (7B8) were purchased From Creative Biolabs (TAB-423MZ-F, USA).

Techniques: Conjugation Assay, Confocal Microscopy, Labeling

Journal: Bioactive Materials

Article Title: Translational selenium nanoparticles trigger apoptosis in triple-negative breast cancer cells through the MAPKs/Bcl2 pathway

doi: 10.1016/j.bioactmat.2026.02.027

Figure Lengend Snippet: In vitro anti-tumor efficacy of PTR-SeNPs and MUC1@PTR-SeNPs on 17 TNBC c ell lines. ( A, B ) Protein expression level of MUC1 in 17 different TNBC cell lines. ( C, D ) IC 50 and maximum % growth inhibition of PTR-SeNPs and MUC1@PTR-SeNPs on 17 TNBC cell lines. ( E, G ) Cell cycle distribution triggered by PTR-SeNPs and MUC1@PTR-SeNPs in HCC1937 and MDA-MB-436 cells. After treatment with PTR-SeNPs or MUC1@PTR-SeNPs (4 and 40 μM) in HCC1937 and MDA-MB-436 cells for 72 h, cells were stained with propidium iodide followed by flow cytometry analysis using MultiCycle software. The apoptotic cell death was quantified by measuring the sub-G1 cell population. ( F, H ) Phosphatidylserine translocation mediated by PTR-SeNPs and MUC1@PTR-SeNPs in HCC1937 and MDA-MB-436 cells. After treatment with MUC1@PTR-SeNPs (4 and 40 μM) for 48 h, cells were co-stained with propidium iodide and Annexin-V-FITC followed by flow cytometry analysis [early apoptotic subset: Annexin V+/PI- (green); late apoptotic subset: Annexin V+/PT+ (red)].

Article Snippet: Anti-human MUC1 therapeutic antibody Fab fragment (7B8) were purchased From Creative Biolabs (TAB-423MZ-F, USA).

Techniques: In Vitro, Expressing, Inhibition, Staining, Flow Cytometry, Software, Translocation Assay

Journal: Bioactive Materials

Article Title: Translational selenium nanoparticles trigger apoptosis in triple-negative breast cancer cells through the MAPKs/Bcl2 pathway

doi: 10.1016/j.bioactmat.2026.02.027

Figure Lengend Snippet: In vivo anti-tumor efficacy of MUC1@PTR- SeNPs. (A) MUC1 mRNA expression in normal tissue and primary breast cancer tumor using GEPIA database. ( B ) MUC1 expression in tumor tissues of MDA-MB-468-bearing mice in preliminary study. (C – E) Dose-dependent study of tumor inhibition effect of MUC1@PTR-SeNPs [75 (Low), 375 (Mid) & 750 μg (High) Se/kg BW/day] on BALB/c nude mice transplanted with MDA-MB-468 xenograft after oral administration for 30 days. PTR-SeNPs (High; 750 μg Se/kg BW/day) was used to investigate the possible improvement of in vivo anti-tumor efficacy by the MUC1@PTR-SeNPs. Quantitative analysis of Se content (μg/g) in (F) blood and (G) tumor tissue of experimental mice. (H) H&E, Ki67 and Tunnel fluorescence staining of tumor sections to detect apoptosis in vivo . (I) Western blot analysis of PARP, p-Bcl-2, Bax and C-caspase-9 protein expression in tumor sections. (J) In the serum of each group of tumor-bearing mice, the results of blood biochemistry-related indexes were analyzed.

Article Snippet: Anti-human MUC1 therapeutic antibody Fab fragment (7B8) were purchased From Creative Biolabs (TAB-423MZ-F, USA).

Techniques: In Vivo, Expressing, Inhibition, Fluorescence, Staining, Western Blot

Journal: Translational Neurodegeneration

Article Title: Transplantation of hiPSC-derived pericytes rescues Alzheimer’s disease phenotypes in APOE 4/4 mice through IGF2-rich apoptotic vesicles

doi: 10.1186/s40035-025-00512-6

Figure Lengend Snippet: IGF2 in ApoVs plays a vital role in functional recovery of APOE 4/4-PCs in vitro. a ApoVs derived from APOE 3/3-PCs or HDFs were subjected to transcriptomic analysis and proteomic analysis. b Principal component analysis (PCA) evaluating the similarities of gene expression profiles between ApoVs −HDFs and ApoVs −PCs . c, d Volcano plot and heatmap of differential gene expression analysis between ApoVs −HDFs and ApoVs −PCs . e – h The differentially expressed genes were subjected to GO enrichment analysis. The GO terms related to neuron protection and cognition ( e ), blood–brain barrier maintenance ( f ), DNA replication and transcription ( g ) and cell growth and metabolic ( h ) were significantly enriched in ApoVs −PCs . i, j Volcano plot ( i ) and heatmap ( j ) of protein expression between ApoVs −HDFs and ApoVs −PCs . k, l qPCR and western blot analysis of IGF2 mRNA expression and protein level in ApoVs −HDFs and ApoVs −PCs . m, n IGF2 knockout in APOE 3/3-PCs using the CRISPR-Cas9 system. IGF2 expression was confirmed by western blot analysis. o, p Flow cytometry analysis with annexin V and PI staining revealed cell death rate in APOE 3/3-PCs, APOE 4/4-PCs, APOE 4/4-PCs + ApoVs −PCs−IGF2KO and APOE 4/4-PCs + ApoVs −PCs−IGF2NC groups ( n = 3 biological repeats for each group). q Representative dextran leakage in APOE 3/3-PCs, APOE 4/4-PCs, APOE 4/4-PCs + ApoVs −PCs−IGF2KO and APOE 4/4-PCs + ApoVs −PCs−IGF2NC groups ( n = 3 biological repeats for each group). r Representative Aβ transcytosis in APOE 3/3-PCs, APOE 4/4-PCs, APOE 4/4-PCs + ApoVs −PCs−IGF2KO and APOE 4/4-PCs + ApoVs −PCs−IGF2NC groups ( n = 3 biological repeats for each group). One-way ANOVA with Tukey’s multiple comparison test; ns, non-significant, * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: The primary antibodies were anti-Aβ 40 (Servicebio, Cat. #GB111197-100, 1:1000), Aβ1-42 Rabbit mAb (ABclonal, Cat. #A24422, 1:1000), phospho-tau-T181 rabbit mAb (ABclonal, Cat. #AP1387, 1:1000, Wuhan, China), and IGF2 (Signalway Antibody, Cat. #32592-2, 1:1000, Frederick, MA).

Techniques: Functional Assay, In Vitro, Derivative Assay, Gene Expression, Expressing, Western Blot, Knock-Out, CRISPR, Flow Cytometry, Staining, Comparison