Journal: Internal Medicine

Article Title: A Hypoglycemia-inducing Giant Borderline Phyllodes Tumor Secreting High-molecular-weight Insulin-Like Growth Factor II: Immunohistochemistry and a Western Blot Analysis

doi: 10.2169/internalmedicine.9287-17

Figure Lengend Snippet: The microscopic findings [Hematoxylin and Eosin (H&E) staining and immunohistochemical staining of the resected tumor]. H&E staining (A) Low-power (×40) and (B) high-power (×400) views showed moderate stromal hypercellularity with mild nuclear atypia and mild pleomorphism of the spindle cells. Focal mildly atypical epithelial hyperplasia was also noted. Stromal overgrowth was absent. Immunohistochemical staining of (C) the phyllodes tumor and (D) normal breast tissue using rabbit polyclonal anti-insulin-like growth factor II (IGF-II) antibodies. The tumor cells, but not the normal breast tissue, were diffusely immunopositive for IGF-II.

Article Snippet: They were probed with rabbit polyclonal anti-IGF-II antibody (Atlas Antibodies, Bromma, Sweden).

Techniques: Staining, Immunohistochemical staining

Journal: Internal Medicine

Article Title: A Hypoglycemia-inducing Giant Borderline Phyllodes Tumor Secreting High-molecular-weight Insulin-Like Growth Factor II: Immunohistochemistry and a Western Blot Analysis

doi: 10.2169/internalmedicine.9287-17

Figure Lengend Snippet: Western blotting of high-molecular-weight IGF-II. Lane 1, serum from a healthy control; lane 2, serum from the patient collected preoperatively; lane 3, serum obtained 3 days after the resection of the tumor; lane 4, phyllodes tumor tissue. Large amounts of high-molecular-weight IGF-II were detected in the serum collected preoperatively (lane 2) and in the tumor tissue (lane 4) but not in serum collected postoperatively (lane 3) or a serum sample from a healthy control (lane 1).

Article Snippet: They were probed with rabbit polyclonal anti-IGF-II antibody (Atlas Antibodies, Bromma, Sweden).

Techniques: Western Blot, High Molecular Weight, Control

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Trial design and vaccine procedures

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Infection, Sterility, Injection

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Multiple sequence alignments of IMP1 and AMA1 from different species. a Multiple alignment of E. stiedae IMP1 with IMP1 proteins from other parasites: Eimeria maxima (UniProt: F4MKA6), Eimeria acervulina (UniProt: U6GHD9), Eimeria tenella (UniProt: F4MKA7), Eimeria mitis (UniProt: U6JVY4), Eimeria praecox (UniProt: U6GBP2); ( b ) multiple alignment of E. stiedae AMA1 with AMA1 proteins from other parasites: Eimeria tenella (UniProt: U6KTA0), Eimeria brunetti (UniProt: U6LBB9), Toxoplasma gondii (UniProt: B6KAM0), Besnoitia besnoiti (UniProt: A0A2A9MBX4), Neospora caninum (UniProt: F0VH85); Blue shading indicates conserved residues. Dashed red boxes represent B-cell epitopes. The transmembrane region is marked with a solid red box

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Sequencing

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: SDS-PAGE and Western blotting analysis of r Es IMP1 ( a ) and r Es AMA1 ( b ). Lane M: Protein molecular weight markers; lanes 1–4: recombinant proteins that were dissolved in the supernatant, 4 M urea, 6 M urea, and 8 M urea after ultrasonication; lane 5: purified recombinant proteins; lane 6: purified recombinant proteins incubated with anti- E. stiedae positive sera; lane 7: purified recombinant proteins incubated with negative sera from coccidia-free rabbits (the bands are indicated using arrows)

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: SDS Page, Western Blot, Molecular Weight, Recombinant, Purification, Incubation

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Protective effects of r Es AMA1 and r Es IMP1 against E. stiedae infection under different evaluation indicators

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Infection

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Gross postmortem examinations of the liver. a PBS-uninfected group; ( b ) PBS-infected group; ( c ) Quil-A-infected group; ( d ) Trx-His-S-infected group; ( e ) r Es AMA1 group; ( f ) r Es IMP1 group

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Infection

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Biochemical estimation of ALT and AST levels in the six groups

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques:

Journal: Parasites & Vectors

Article Title: Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits

doi: 10.1186/s13071-022-05492-4

Figure Lengend Snippet: Changes in serum antibody levels. The changes in serum anti-r Es IMP1 ( a ) and r Es AMA1 ( b ) antibody levels after the first vaccination (week 0, indicated by arrows), booster vaccination (week 2, indicated by arrows), and infection with E. stiedae (week 4, indicated by an arrow)

Article Snippet: The separated r Es AMA1 and r Es IMP1 were transferred onto nitrocellulose (NC) membranes (Boster, Wuhan, China), separately.

Techniques: Infection

Journal: Microorganisms

Article Title: Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

doi: 10.3390/microorganisms9071537

Figure Lengend Snippet: Primers sequences for qPCR.

Article Snippet: IGF2 , Rabbit , 1/500 , OAAB07463 , OAAB07463 , BosterBio , 20.

Techniques: Amplification

Journal: Microorganisms

Article Title: Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

doi: 10.3390/microorganisms9071537

Figure Lengend Snippet: Antibodies used for Western blot.

Article Snippet: IGF2 , Rabbit , 1/500 , OAAB07463 , OAAB07463 , BosterBio , 20.

Techniques: Western Blot

Journal: Microorganisms

Article Title: Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

doi: 10.3390/microorganisms9071537

Figure Lengend Snippet: Transcription factors and autoantigens transcripts in the whole thymus. Complementary DNA obtained from mock- and CV-B4-infected thymuses ( n = 5–7), harvested at different time-points, was submitted to quantification of Aire , Fezf2 , Igf2, and Myo7 transcripts by qPCR. Transcripts levels were normalized to those of Oaz1. Relative gene expression in CV-B4 E2- vs. mock-infected thymuses was calculated, as described in , and expressed as 100 × mean 2 −ΔΔCt values ± SD. The unpaired t -test was used for statistical analysis. ** p < 0.01. Mock (●) mock-infected thymuses harvested at different time points, from fetuses at day 17 of gestation (day 17G), and newborns at day 1 and day 5 from birth. CV-B4 10G (▼): thymus harvested from mice born to dams inoculated with CV-B4 at day 10 of gestation; CV-B4 17G (♦): thymus harvested from mice born to dams inoculated with CV-B4 at day 17 of gestation.

Article Snippet: IGF2 , Rabbit , 1/500 , OAAB07463 , OAAB07463 , BosterBio , 20.

Techniques: Immunopeptidomics, Infection, Gene Expression

Journal: Microorganisms

Article Title: Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

doi: 10.3390/microorganisms9071537

Figure Lengend Snippet: Transcription factors and autoantigens transcripts in enriched thymic epithelial cells. Whole thymuses were harvested, at different time-points, from offspring born to mock- and CV-B4-infected dams. Thymuses from fetuses or neonates of the same litter ( n = 5–12) were pooled and subjected to TECs isolation. Transcripts levels for Aire , Fezf2 , Igf2, and Myo7 , in enriched TECs, were determined by RT-qPCR and normalized to those of Oaz1. Relative gene expression in TECs from CV-B4 E2- vs. mock-infected thymuses was calculated, as described in , and expressed as 100 × mean 2 −ΔΔCt values ± SD. The unpaired t -test was used for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001. Mock (●) TECs from mock-infected thymuses harvested at different time points, from fetuses at day 17 of gestation (day 17G), and newborns at day 1 and day 5 from birth. CV-B4 10G (▼): TECs from thymus harvested from mice born to dams inoculated with CV-B4 at day 10 of gestation; CV-B4 17G (♦): thymus harvested from mice born to dams inoculated with CV-B4 at day 17 of gestation.

Article Snippet: IGF2 , Rabbit , 1/500 , OAAB07463 , OAAB07463 , BosterBio , 20.

Techniques: Immunopeptidomics, Infection, Isolation, Quantitative RT-PCR, Gene Expression

Journal: Microorganisms

Article Title: Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

doi: 10.3390/microorganisms9071537

Figure Lengend Snippet: Transcription factors and autoantigens protein expression in the whole thymus. ( a ) Representative blots depicting the expression levels of AIRE, FEZF2, IGF2, and MYO7 proteins in CV-B4- and mock-infected thymus sampled at day 1. ( b ) Relative expression of AIRE, FEZF2, IGF2, and MYO7 determined by Western blot analysis. Proteins obtained from mock- and CV-B4-infected thymuses ( n = 3–5), harvested at different time-points, were submitted to AIRE, FEZF2, IGF2, and MYO7 quantification by Western-blot analysis. Proteins levels were normalized to those of VINCULIN used as a loading control. Relative protein levels in CV-B4 E2- vs. mock-infected thymuses are represented as mean ± SD. The unpaired t -test was used for statistical analysis. * p < 0.05. Mock (●): mock-infected thymuses harvested at different time points, from fetuses at day 17 of gestation (day 17G), and newborns at day 1 and day 5 from birth. CV-B4 10G (▼): thymus harvested from mice born to dams inoculated with CV-B4 at day 10 of gestation; CV-B4 17G (♦): thymus harvested from mice born to dams inoculated with CV-B4 at day 17 of gestation.

Article Snippet: IGF2 , Rabbit , 1/500 , OAAB07463 , OAAB07463 , BosterBio , 20.

Techniques: Immunopeptidomics, Expressing, Infection, Western Blot, Control

Journal: Microorganisms

Article Title: Coxsackievirus B4 Transplacental Infection Severely Disturbs Central Tolerogenic Mechanisms in the Fetal Thymus

doi: 10.3390/microorganisms9071537

Figure Lengend Snippet: Relationship between viral load and transcription factors, Aire ( a ) and Fezf2 ( b ), and autoantigens transcripts, Igf2 ( c ) and Myo7 ( d ), in the thymus. For each harvested thymus (CV-B4-infected or age-matched control), transcription factors and autoantigens transcripts together with the corresponding viral load were measured and plotted in the same graph. To better assess the effect of infection on transcription factors and autoantigens expression, an eventual correlation between both parameters was evaluated using the Spearmen’s correlation test. r is the Spearman’s correlation coefficient. p -values were corrected according to the Benjamini–Hochberg procedure ( P BH ). Thymuses harvested at day 17G (Day 17G (10G) (●)), day 1 (Day 1 (10G) (▲)) or day 5 (Day 5 (10G) (♦)) from mice born to dams inoculated (or mock) with CV-B4 at day 10 of gestation; thymuses harvested at day 1 (Day 1 (17G) (▼)) or day 5 (Day 5 (17G) (■)) from mice born to dams inoculated (or mock) with CV-B4 at day 17 of gestation. x and y axis were log-scaled.

Article Snippet: IGF2 , Rabbit , 1/500 , OAAB07463 , OAAB07463 , BosterBio , 20.

Techniques: Immunopeptidomics, Infection, Control, Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The primers and TaqMan probes of IGF, ER genes used for RT-PCR

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques:

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The mRNA levels of IGF-1, IGF-1R, IGF-2, IGF-2R, IGFBP-3, ERα and ERβ in EAC, tumor-adjacent, and control groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Control

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The immunostaining of IGF-1R, IGF-2R, ERα and ERβ in EAC, atypical hyperplasia, and control groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Immunostaining, Control, Positive Control

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The relationship between the IGF and ER mRNA expression and clinico-pathological features of endometrioid adenocarcinoma

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The relationship between the IGF-1R, IGF2R, ERα and ERβ protein expression and clinico-pathological features of endometrioid adenocarcinoma

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: Correlation analysis of IGF and ER mRNA expression with clinico-pathological features of EAC

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: Correlation analysis of IGF-1R, IGF-2R, and ERα and ERβ protein expression with clinico-pathological features of EAC

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing

Journal: BMC Cancer

Article Title: Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

doi: 10.1186/1471-2407-12-262

Figure Lengend Snippet: The analysis of the correlation between the mRNA expression of IGFs with ER subtypes in three groups

Article Snippet: Bio-engineering Co ., Ltd ., Wuhan , China), IGF-2R (polyclonal rabbit anti-human IGF-2R, 1:50 dilution, Wuhan Boster .

Techniques: Expressing, Control

Journal: Bioactive Materials

Article Title: Translational selenium nanoparticles trigger apoptosis in triple-negative breast cancer cells through the MAPKs/Bcl2 pathway

doi: 10.1016/j.bioactmat.2026.02.027

Figure Lengend Snippet: Schematic illustration of PTR-SeNPs and MUC1@PTR-SeNPs synthesis and their anti-tumor efficacy against human triple-negative breast cancer.

Article Snippet: Anti-human MUC1 therapeutic antibody Fab fragment (7B8) were purchased From Creative Biolabs (TAB-423MZ-F, USA).

Techniques:

Journal: Bioactive Materials

Article Title: Translational selenium nanoparticles trigger apoptosis in triple-negative breast cancer cells through the MAPKs/Bcl2 pathway

doi: 10.1016/j.bioactmat.2026.02.027

Figure Lengend Snippet: Structure characterization of PTR-SeNPs and MUC1@ PTR-SeNPs. Structure characterization of PTR-SeNPs by (A) TEM, (B) Zetasizer Nano ZS, (C, D) Nanosight NS300, (E1-4) HRTEM-EDS and (F, G) FT-IR. (H) Confirmation of MUC1-C + PTR-SeNPs conjugation by confocal microscopy after fluorescent labeling with anti-mouse IgG (H + L). (I, J) Characterization results of the particle size and potential of MUC1@PTR-SeNPs

Article Snippet: Anti-human MUC1 therapeutic antibody Fab fragment (7B8) were purchased From Creative Biolabs (TAB-423MZ-F, USA).

Techniques: Conjugation Assay, Confocal Microscopy, Labeling

Journal: Bioactive Materials

Article Title: Translational selenium nanoparticles trigger apoptosis in triple-negative breast cancer cells through the MAPKs/Bcl2 pathway

doi: 10.1016/j.bioactmat.2026.02.027

Figure Lengend Snippet: In vitro anti-tumor efficacy of PTR-SeNPs and MUC1@PTR-SeNPs on 17 TNBC c ell lines. ( A, B ) Protein expression level of MUC1 in 17 different TNBC cell lines. ( C, D ) IC 50 and maximum % growth inhibition of PTR-SeNPs and MUC1@PTR-SeNPs on 17 TNBC cell lines. ( E, G ) Cell cycle distribution triggered by PTR-SeNPs and MUC1@PTR-SeNPs in HCC1937 and MDA-MB-436 cells. After treatment with PTR-SeNPs or MUC1@PTR-SeNPs (4 and 40 μM) in HCC1937 and MDA-MB-436 cells for 72 h, cells were stained with propidium iodide followed by flow cytometry analysis using MultiCycle software. The apoptotic cell death was quantified by measuring the sub-G1 cell population. ( F, H ) Phosphatidylserine translocation mediated by PTR-SeNPs and MUC1@PTR-SeNPs in HCC1937 and MDA-MB-436 cells. After treatment with MUC1@PTR-SeNPs (4 and 40 μM) for 48 h, cells were co-stained with propidium iodide and Annexin-V-FITC followed by flow cytometry analysis [early apoptotic subset: Annexin V+/PI- (green); late apoptotic subset: Annexin V+/PT+ (red)].

Article Snippet: Anti-human MUC1 therapeutic antibody Fab fragment (7B8) were purchased From Creative Biolabs (TAB-423MZ-F, USA).

Techniques: In Vitro, Expressing, Inhibition, Staining, Flow Cytometry, Software, Translocation Assay

Journal: Bioactive Materials

Article Title: Translational selenium nanoparticles trigger apoptosis in triple-negative breast cancer cells through the MAPKs/Bcl2 pathway

doi: 10.1016/j.bioactmat.2026.02.027

Figure Lengend Snippet: In vivo anti-tumor efficacy of MUC1@PTR- SeNPs. (A) MUC1 mRNA expression in normal tissue and primary breast cancer tumor using GEPIA database. ( B ) MUC1 expression in tumor tissues of MDA-MB-468-bearing mice in preliminary study. (C – E) Dose-dependent study of tumor inhibition effect of MUC1@PTR-SeNPs [75 (Low), 375 (Mid) & 750 μg (High) Se/kg BW/day] on BALB/c nude mice transplanted with MDA-MB-468 xenograft after oral administration for 30 days. PTR-SeNPs (High; 750 μg Se/kg BW/day) was used to investigate the possible improvement of in vivo anti-tumor efficacy by the MUC1@PTR-SeNPs. Quantitative analysis of Se content (μg/g) in (F) blood and (G) tumor tissue of experimental mice. (H) H&E, Ki67 and Tunnel fluorescence staining of tumor sections to detect apoptosis in vivo . (I) Western blot analysis of PARP, p-Bcl-2, Bax and C-caspase-9 protein expression in tumor sections. (J) In the serum of each group of tumor-bearing mice, the results of blood biochemistry-related indexes were analyzed.

Article Snippet: Anti-human MUC1 therapeutic antibody Fab fragment (7B8) were purchased From Creative Biolabs (TAB-423MZ-F, USA).

Techniques: In Vivo, Expressing, Inhibition, Fluorescence, Staining, Western Blot